Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Matrix-assisted cell transfer for intervertebral disc cell therapy.

Helge Bertram1, Markus Kroeber, Haili Wang

  • 1Division of Experimental Orthopaedics, Orthopaedic University Hospital Heidelberg, D-69118 Heidelberg, Germany.

Biochemical and Biophysical Research Communications
|May 11, 2005
PubMed
Summary

Intervertebral disc (IVD) cell therapy shows promise for disc regeneration. Optimized fibrin matrix and reduced intradiscal pressure improve cell survival and transfer efficiency, but advanced substitutes are needed for the low-nutrient environment.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Double-Antibody Sandwich ELISA for the Detection of HPV16 E6 Protein.

Diagnostics (Basel, Switzerland)·2026
Same author

Drought Severity and Nitrogen Addition Interactively Modulate Seedling Growth and Resource-Use Strategies of <i>Quercus wutaishanica</i>.

Biology·2026
Same author

Multi-Trait Genetic Insights Into Schizophrenia Across Ancestries: Genome-Wide Association Meta-Analyses, Machine Learning, and Drug Repurposing Study.

Brain and behavior·2026
Same author

[Dupuytren's disease - a fibroproliferative systemic disorder].

MMW Fortschritte der Medizin·2026
Same author

Loss of PDL1 in mesenchymal stromal cells from young-onset MDS-RAEB-1 promotes inflamm-aging through epigenetic dysregulation of H3K9me3.

Stem cells translational medicine·2026
Same author

[Palmar approach for silicone interposition arthroplasty of the finger middle joint].

Operative Orthopadie und Traumatologie·2026

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Orthopedics

Background:

  • Intervertebral disc (IVD) degeneration is a significant cause of low back pain.
  • Cell therapy offers a potential strategy for IVD regeneration.
  • Challenges include efficient cell delivery and survival within the avascular IVD environment.

Purpose of the Study:

  • To evaluate the efficacy of cell therapy for IVD regeneration.
  • To assess cell transfer efficiency and survival using a novel fibrin matrix.
  • To identify factors influencing cell viability in the IVD.

Main Methods:

  • Recombinant cells were injected into rabbit IVDs using a two-component fibrin matrix.
  • Cell survival and distribution were quantified via luciferase expression in vitro, in situ, and in vivo.

Related Experiment Videos

  • IVDs were subjected to nucleotomy or mechanical degeneration.
  • Fibrin matrix was enriched with serum biomolecules.

    • Main Results:

    • 35-50% of matrix-injected cells remained in the nucleus and transition zone, unlike medium-injected cells.
    • Nucleotomy was critical for cell survival beyond 3 days.
    • Nutritional enrichment of the fibrin matrix significantly enhanced cell viability.

    Conclusions:

    • A fibrin matrix improves cell retention in IVDs compared to simple injection.
    • Reducing intradiscal pressure via nucleotomy is crucial for cell survival.
    • Advanced matrix substitutes with enhanced nutritional support are required for effective IVD cell therapy.