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L7/Pcp-2-specific expression of Cre recombinase using knock-in approach

Hiromitsu Saito1, Hideki Tsumura, Seiichi Otake

  • 1Department of Animal Genomics, Functional Genomics Institute, Mie University Life Science Research Center, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.

Biochemical and Biophysical Research Communications
|May 11, 2005
PubMed

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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