Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymorphisms in human pre-miRNAs.

Naoharu Iwai1, Hiroaki Naraba

  • 1National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. iwai@ri.ncvc.go.jp

Biochemical and Biophysical Research Communications
|May 11, 2005
PubMed
Summary

Genetic variations in microRNAs (miRNAs) can impact gene regulation. Researchers identified a specific polymorphism in mature miR-30c-2, potentially altering its function and biological effects.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Withdrawal: Microsomal prostaglandin E synthase-1 is overexpressed in inflammatory bowel disease: Evidence for involvement of the transcription factor Egr-1.

The Journal of biological chemistry·2020
Same author

Absence of miR-182 Augments Cardiac Allograft Survival.

Transplantation·2016
Same author

miR-182 Regulates Metabolic Homeostasis by Modulating Glucose Utilization in Muscle.

Cell reports·2016
Same author

A positive feedback loop between progesterone and microsomal prostaglandin E synthase-1-mediated PGE2 promotes production of both in mouse granulosa cells.

Prostaglandins & other lipid mediators·2016
Same author

Loss of miR-182 affects B-cell extrafollicular antibody response.

Immunology·2016
Same author

Establishment of the MethyLight Assay for Assessing Aging, Cigarette Smoking, and Alcohol Consumption.

BioMed research international·2015

Area of Science:

  • Genetics
  • Molecular Biology
  • Non-coding RNA research

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression through translational repression.
  • miRNAs originate as pri-miRNAs and are processed into pre-miRNAs before becoming mature miRNAs.
  • The functional impact of genetic variations within miRNA precursor regions remains largely unexplored.

Purpose of the Study:

  • To investigate the presence and potential functional consequences of polymorphisms in human pre-miRNA and mature miRNA sequences.
  • To explore how these genetic variations might influence miRNA processing and target selection.

Main Methods:

  • Sequencing of 173 human pre-miRNA genome regions across 96 individuals.
  • Analysis of identified polymorphisms for their potential effects on miRNA processing and function.

Main Results:

  • Discovery of 10 polymorphisms within 10 pre-miRNA hairpin regions.
  • Identification of a specific C to A polymorphism in the mature miR-30c-2 sequence.
  • Most identified polymorphisms did not appear to affect miRNA processing, but the miR-30c-2 variant may impact target selection.

Conclusions:

  • This study provides the first report of polymorphisms within pre-miRNA sequences.
  • A specific pre-miRNA polymorphism in miR-30c-2 has the potential to alter miRNA target selection and exert significant biological effects.
  • Further research is warranted to elucidate the functional implications of miRNA polymorphisms.

Related Experiment Videos