Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Insights into TOR function and rapamycin response: chemical genomic profiling by using a high-density cell array

Michael W Xie1, Fulai Jin, Heejun Hwang

  • 1Department of Molecular, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.

Proceedings of the National Academy of Sciences of the United States of America
|May 11, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid reduction in global chromatin loop size after acute STAG2 reconstitution in human cancer cells.

The Journal of biological chemistry·2026
Same author

Rapid reduction in global chromatin loop size after acute STAG2 reconstitution in human cancer cells.

bioRxiv : the preprint server for biology·2026
Same author

Genetic correlation analysis of Alzheimer's disease and stroke implicates PHLPP1 as a shared locus in individuals of African ancestry.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2026
Same author

Proinsulin regulators identified with CRISPR screen and in vivo mouse QTL mapping.

Nature communications·2026
Same author

Enhanced Hi-C Capture Analysis reveals complex regulatory architecture at the <i>PICALM_EED</i> locus for Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Researchers developed a high-density cell array for rapid, large-scale functional genomics. This tool identified gene deletions improving cell fitness with rapamycin, offering insights into neurodegenerative diseases and cancer therapies.

Area of Science:

  • Genomics
  • Cell Biology
  • Pharmacology

Background:

  • Complete genome sequences enable large-scale functional analyses in biology and medicine.
  • Understanding genotype-dependent drug responses is crucial for targeted therapies.

Purpose of the Study:

  • To develop a high-density cell array for efficient genomewide functional analysis.
  • To map gene-fitness relationships in response to rapamycin.
  • To investigate the distinct functions of TOR1 and TOR2 in yeast.

Main Methods:

  • Development of a high-density cell array for quantitative, automated readout of cell fitness.
  • Screening of the Saccharomyces cerevisiae gene-deletion library using the cell array.
  • Analysis of gene-fitness interactions with rapamycin and functional mapping of TOR proteins.

Related Experiment Videos

Main Results:

  • Identified a class of gene deletions that enhance cell fitness in the presence of rapamycin.
  • Demonstrated distinct rapamycin-sensitive functions between TOR1 and TOR2, mapping the difference to an N-terminal region.
  • Provided insights into potential therapeutic applications of rapamycin for neurodegenerative diseases and cancer.

Conclusions:

  • The high-density cell array facilitates efficient genomewide functional screening.
  • Rapamycin response is complex and genotype-dependent, with implications for pharmacogenomics.
  • Findings suggest novel therapeutic strategies for diseases involving TOR signaling and highlight the distinct roles of TOR1 and TOR2.