Dynamics of protein recovery from process filtration systems using microporous membrane filter cartridges

The purpose of this report is to address the concern for protein concentration recovery through membrane filter cartridges at the process scale. Filtration systems consisting of pre- and/or final membrane filter cartridges were evaluated for protein concentration recovery under typical manufacturing conditions of continuous flow and high throughput volumes. Results of the study conclusively demonstrate that consideration of the protein adsorptive properties and recovery performance of cartridge filters can provide efficient bio-burden and particulate control without compromising protein yields. Understanding the dynamics of protein recovery through various prefilter and final filter cartridges can play an important role in the proper selection of a filtration system to ensure optimal life and protein yield when filtering dilute protein solutions.