Related Experiment Video
Updated: Aug 18, 2026
A Small Animal Model of Ex Vivo Normothermic Liver Perfusion
Published on: June 27, 2018
[Functional and morphological study of the local and systemic hypothermia on dog's liver]
Venilton José Siqueira1, Murched Omar Taha, Djalma José Fagundes
1Disciplina de Técnica Operatória e Cirurgia Experimental Universidade Federal de São Paulo--Escola Paulista de Medicina.
Purpose:
To compare hepatic lesions produced by two types of hypothermia; the systemic and the local or topic.
Methods:
Twenty dogs distributed in two groups were studied: the first submitted to local hypothermia and the second to systemic hypothermia. In all groups, biochemical dosages for alanina allytransferase (A.L.T.), aspartate aminotrasnferase (A.S.T.) and direct bilirubin (T.D.), conventional optical microscopy and electronic transmission microscopy were performed in times T0, Test, and T60, that is, before the hypothermia (T0), after temperature stabilization at 10 degrees lower than initial temperature (Test), and after sixty minutes of hypothermia (T60).
Results:
The data analysis, both of the biochemical profile and of the microscopy showed that in the group of animals with selective hypothermia, the hepatic lesions were more intense when compared to the systemic hypothermia group.
Conclusion:
The selective hypothermia causes more lesions to the liver than the systemic.
