Related Experiment Videos
Chitosan/beta-lactoglobulin core-shell nanoparticles as nutraceutical carriers
Lingyun Chen1, Muriel Subirade
1Chaire de recherche du Canada sur les protéines, Institut de recherche sur les nutraceutiques et les aliments fonctionnels (INAF/STELA), Université Laval, Pavillon Paul Comtois, Sainte-Foy, Qué., Canada.
Biomaterials
|May 12, 2005
Summary
Chitosan/beta-lactoglobulin nanoparticles show promise for oral nutraceutical delivery. Native beta-lactoglobulin shells offer superior resistance to simulated gastric conditions compared to denatured versions.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Food Science
Background:
- Developing effective oral drug delivery systems is crucial for nutraceuticals.
- Biocompatible carriers are needed to protect sensitive compounds during gastrointestinal transit.
- Chitosan and beta-lactoglobulin offer unique properties for nanoparticle formulation.
Purpose of the Study:
- To prepare and characterize chitosan/beta-lactoglobulin (CS-betalg) core-shell nanoparticles.
- To investigate the influence of pH and beta-lactoglobulin (betalg) properties on nanoparticle characteristics.
- To evaluate the stability and release profile of these nanoparticles under simulated gastrointestinal conditions.
Main Methods:
- Nanoparticles were synthesized using ionic gelation with sodium tripolyphosphate.
- Zeta potential, loading efficiency, and adsorption mechanisms were analyzed.
- Brilliant blue release studies were conducted under simulated gastric and intestinal conditions.
Main Results:
- Uniform CS-betalg nanoparticles with positive surface charge (20-60 mV zeta potential) were successfully prepared.
- Loading efficiency (1-60%) was highly dependent on formulation pH and betalg state (native vs. denatured).
- Native betalg shells provided significant resistance to acid and pepsin, while denatured betalg showed less stability.
Conclusions:
- CS-betalg nanoparticles are a viable biocompatible carrier for oral nutraceutical delivery.
- Native betalg shells enhance nanoparticle stability in the stomach, protecting payload.
- The shell integrity is compromised in simulated intestinal conditions by pancreatin, allowing for controlled release.