Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein sequence tags: a novel solution for comparative proteomics.

Karsten Kuhn1, Thorsten Prinz, Jürgen Schäfer

  • 1Proteome Sciences R&D, Frankfurt am Main, Germany.

Proteomics
|May 12, 2005
PubMed
Summary

Protein Sequence Tag (PST) technology enables broad proteome profiling, detecting diverse proteins like membrane and nuclear types. Its quantitative version offers a powerful alternative for mass spectrometry-based protein analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoscopic retrograde cholangiopancreatography as a diagnostic key tool for biliary atresia: feasibility, safety and accuracy in neonates with cholestatic liver disease.

European journal of pediatrics·2025
Same author

The role of electrode placement in subthalamic nucleus deep brain stimulation for improving gait in Parkinson's Disease.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2025
Same author

Brain Drain for Brain Gain: Potential Applications of Robotic-assisted Lymphatic Microsurgery in the Management of Neurological Disorders.

Plastic and reconstructive surgery. Global open·2025
Same author

White paper on the current state of imaging biomarkers in paediatric oncology.

Pediatric radiology·2025
Same author

Correction: GPOH Guidelines for Diagnosis and First-line Treatment of Patients with Neuroblastic Tumors, update 2025.

Klinische Padiatrie·2025
Same author

GPOH Guidelines for Diagnosis and First-line Treatment of Patients with Neuroblastic Tumors, update 2025.

Klinische Padiatrie·2025

Area of Science:

  • Proteomics
  • Biotechnology
  • Analytical Chemistry

Background:

  • Comparative proteome profiling is crucial for understanding biological systems.
  • Stable isotope labeling and mass spectrometry are established techniques.
  • Identifying a wide range of proteins, including challenging membrane and nuclear proteins, remains an area of development.

Purpose of the Study:

  • To demonstrate the potential of a proprietary Protein Sequence Tag (PST) technology for proteome profiling.
  • To highlight PST's capability in detecting diverse protein classes.
  • To introduce the quantitative PST version for enhanced analytical power.

Main Methods:

  • Utilizing proprietary Protein Sequence Tag (PST) technology.
  • Employing mass spectrometry for protein identification and quantification.

Related Experiment Videos

  • Comparing PST approach with multidimensional protein identification technology (MIP-T).

    • Main Results:

    • PST technology effectively detects a broad spectrum of proteins.
    • PST demonstrates efficacy in identifying pharmaceutically relevant membrane and nuclear proteins.
    • The quantitative PST version provides comparative protein analysis similar to MIP-T but with added quantitative capabilities.

    Conclusions:

    • PST technology offers a versatile and powerful approach for comparative proteome profiling.
    • PST is particularly advantageous for its ability to detect challenging protein types.
    • The quantitative PST version enhances its utility for comprehensive proteomic studies.