Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oxazine 170 induces DNA:RNA:DNA triplex formation.

Guangtao Song1, Feifei Xing, Xiaogang Qu

  • 1Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China.

Journal of Medicinal Chemistry
|May 13, 2005
PubMed
Summary

The oxazine 170 dye induces formation of a poly rA:(poly dT)(2) hybrid triplex structure. This dye selectively binds to the triplex, offering potential for designing new triplex-modulating compounds.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrical impedance spectroscopy of young and old mouse multiple tissues.

Biomedical physics & engineering express·2026
Same author

Machine learning model for predicting rebleeding risk after endoscopic variceal ligation in esophageal variceal bleeding.

Scientific reports·2026
Same author

Synergistic amelioration of cholestatic liver fibrosis by combined total astragalus saponins and AAV8.<i>Numb</i>-Exon3 through modulation of hepatic progenitor cell differentiation.

Frontiers in pharmacology·2026
Same author

A combination nano-immunotherapy targeting cholesterol crystals and STING signaling enhanced disruption atherosclerotic plaque pathogenesis.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Lanthanide Perovskite Heterostructures with Stabilized Dual-Mode Luminescence for Multilevel Information Encryption.

ACS nano·2026
Same author

Mechanical force regulates the inhibitory function of PD-1.

EMBO reports·2026

Area of Science:

  • Molecular biology
  • Biophysical chemistry
  • Dye chemistry

Background:

  • DNA and RNA triplex structures are important in molecular biology.
  • Controlling triplex formation is crucial for various applications.
  • Oxazine dyes are known for their fluorescent properties.

Purpose of the Study:

  • To investigate the effect of oxazine 170 on poly rA:(poly dT)(2) triplex formation.
  • To understand the mechanism of oxazine 170-induced triplex stabilization.
  • To explore oxazine 170 as a lead compound for triplex-modulating agents.

Main Methods:

  • Spectroscopic analysis of nucleic acid structures.
  • Solution-based biophysical assays.
  • Chemical synthesis and characterization of oxazine dyes.

Related Experiment Videos

Main Results:

  • Oxazine 170 induced the formation of a poly rA:(poly dT)(2) hybrid triplex.
  • This induction occurred under conditions where the triplex would not normally form.
  • Structural selective binding of oxazine 170 to the triplex was identified as the driving force.

Conclusions:

  • Oxazine 170 can stabilize and induce the formation of specific nucleic acid triplex structures.
  • The dye's selective binding mechanism provides a basis for designing novel triplex-targeting molecules.
  • This research opens avenues for developing new tools in molecular biology and drug discovery.