Related Experiment Videos
Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging
P G Smith1, F Oakley, M Fernandez
1School of Biochemistry & Microbiology, University of Leeds, Leeds, UK.
Gene Therapy
|May 13, 2005
Summary
Herpesvirus saimiri (HVS) vectors efficiently infect cells, maintaining their genome as episomes for stable, long-term transgene expression in vivo. This suggests potential for treating liver diseases.
Area of Science:
- Virology
- Gene Therapy
- Molecular Biology
Background:
- Herpesvirus saimiri (HVS) is a versatile viral vector.
- HVS vectors stably transduce dividing cells via nonintegrated episomes.
- Sustained transgene expression is achievable both in vitro and in vivo.
Purpose of the Study:
- To evaluate the in vivo dissemination and biodistribution of HVS-based vectors.
- To assess the duration and stability of transgene expression mediated by HVS vectors.
- To explore the potential of HVS vectors for therapeutic applications, particularly in liver diseases.
Main Methods:
- Intravenous and intraperitoneal administration of HVS-based vectors in mice.
- Bioluminescence imaging to track vector dissemination and transgene expression.
- Analysis of viral genome persistence and tissue tropism.
Main Results:
- HVS vectors successfully infected various mouse tissues, establishing persistent latent infections.
- Sustained luciferase expression was observed for 10 weeks due to stable episomal maintenance.
- High levels of transgene expression were detected in the liver, with infection of hepatic stellate cells.
Conclusions:
- HVS-based vectors demonstrate efficient in vivo dissemination and stable episomal persistence.
- The long-term transgene expression capability of HVS vectors is confirmed.
- HVS vectors show promise for gene therapy of inherited and acquired liver diseases.