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Related Experiment Videos

Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging.

P G Smith1, F Oakley, M Fernandez

  • 1School of Biochemistry & Microbiology, University of Leeds, Leeds, UK.

Gene Therapy
|May 13, 2005
PubMed
Summary

Herpesvirus saimiri (HVS) vectors efficiently infect cells, maintaining their genome as episomes for stable, long-term transgene expression in vivo. This suggests potential for treating liver diseases.

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Area of Science:

  • Virology
  • Gene Therapy
  • Molecular Biology

Background:

  • Herpesvirus saimiri (HVS) is a versatile viral vector.
  • HVS vectors stably transduce dividing cells via nonintegrated episomes.
  • Sustained transgene expression is achievable both in vitro and in vivo.

Purpose of the Study:

  • To evaluate the in vivo dissemination and biodistribution of HVS-based vectors.
  • To assess the duration and stability of transgene expression mediated by HVS vectors.
  • To explore the potential of HVS vectors for therapeutic applications, particularly in liver diseases.

Main Methods:

  • Intravenous and intraperitoneal administration of HVS-based vectors in mice.
  • Bioluminescence imaging to track vector dissemination and transgene expression.

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  • Analysis of viral genome persistence and tissue tropism.

    • Main Results:

    • HVS vectors successfully infected various mouse tissues, establishing persistent latent infections.
    • Sustained luciferase expression was observed for 10 weeks due to stable episomal maintenance.
    • High levels of transgene expression were detected in the liver, with infection of hepatic stellate cells.

    Conclusions:

    • HVS-based vectors demonstrate efficient in vivo dissemination and stable episomal persistence.
    • The long-term transgene expression capability of HVS vectors is confirmed.
    • HVS vectors show promise for gene therapy of inherited and acquired liver diseases.