Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A method for the preparation of normalized cDNA libraries enriched with full-length sequences].

P A Zhulidov, E A Bogdanova, A S Shcheglov

    Bioorganicheskaia Khimiia
    |May 14, 2005
    PubMed
    Summary

    Researchers created a novel method for normalizing complementary DNA (cDNA) libraries using a crab enzyme. This technique efficiently enriches for full-length sequences, simplifying molecular biology workflows.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A Bacterial Bioluminescent System as a Tool for Monitoring Biogenic Aldehydes.

    Doklady. Biochemistry and biophysics·2026
    Same author

    Assessment of the preventive effect of knockdown of cellular genes <i>NXF1</i>, PRPS1<i>PRPS1</i> and <i>NAA10</i> in influenza infection in an <i>in vitro</i> model.

    Voprosy virusologii·2025
    Same author

    Distinct organization of adaptive immunity in the long-lived rodent Spalax galili.

    Nature aging·2023
    Same author

    Alternative Methods of Evaluation of Antimicrobial Activity of Breast Milk Serum.

    Bulletin of experimental biology and medicine·2021
    Same author

    The Recombinant Luciferase of the Fungus Neonothopanus nambi: Obtaining and Properties.

    Doklady. Biochemistry and biophysics·2021
    Same author

    [Increasing the Uniformity of Genome Fragment Coverage for High-Throughput Sequencing of Influenza A Virus].

    Molekuliarnaia biologiia·2020

    Area of Science:

    • Molecular Biology
    • Enzymology
    • Genomics

    Context:

    • Developing normalized complementary DNA (cDNA) libraries is crucial for accurate gene expression analysis.
    • Traditional normalization methods often involve laborious and inefficient physical separation techniques.
    • Recent advancements include the characterization of novel enzymes with specific DNA-cleaving properties.

    Purpose:

    • To introduce a new, efficient method for preparing normalized cDNA libraries enriched with full-length sequences.
    • To leverage the properties of a thermostable duplex-specific nuclease from the Kamchatka crab for cDNA normalization.
    • To eliminate the need for cumbersome physical separation steps in cDNA library preparation.

    Summary:

    • A novel cDNA normalization method utilizes a thermostable duplex-specific nuclease (DSN) from the Kamchatka crab.

    Related Experiment Videos

  • DSN specifically cleaves double-stranded DNA while remaining inactive towards single-stranded DNA.
  • This enzymatic approach enables efficient normalization of cDNA samples, enriching for full-length sequences without physical separation.

    • Impact:

    • Simplifies and enhances the process of creating normalized cDNA libraries.
    • Improves the yield of full-length cDNA sequences, crucial for downstream applications.
    • Demonstrated efficiency in model experiments using human tissue cDNA samples, indicating broad applicability.