Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-sensitivity ion mobility spectrometry/mass spectrometry using electrodynamic ion funnel interfaces.

Keqi Tang1, Alexandre A Shvartsburg, Hak-No Lee

  • 1Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P.O. Box 999, Richland, Washington 99352, USA.

Analytical Chemistry
|May 14, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Research on Thermal Runaway Monitoring Methods for Lithium-Ion Batteries Based on Continuous Acoustic Emission Technology.

Sensors (Basel, Switzerland)·2026
Same author

Improved Deep Learning Prediction of TCR-HLA Associations.

Statistics in biosciences·2026
Same author

Digital Revitalization of a Legacy Linear Ion Trap System.

Analytical chemistry·2026
Same author

Janus-Type Electrostatic Potential Gradient-Activated Dynamic Zn<sup>2+</sup>-Coordinating Nitrogen Sites in Molecularly Locked Nanocellulose Separators for Stable Zinc-Ion Batteries.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

CARDIAC-FM: A Multimodal Foundation Model for Cardiovascular Risk Prediction Using ECG and Cardiac MRI.

medRxiv : the preprint server for health sciences·2026
Same author

Ni-Atom Induced Interface Water Reorientation around Ru Clusters for Alkaline Hydrogen Evolution Reaction.

Small (Weinheim an der Bergstrasse, Germany)·2026

We developed a new electrospray ionization-ion mobility spectrometry-mass spectrometry (ESI-IMS-MS) instrument with electrodynamic ion funnels. This significantly improves ion transmission and sensitivity, making IMS-MS a powerful tool for complex mixture analysis.

Area of Science:

  • Analytical Chemistry
  • Physical Chemistry
  • Biophysics

Background:

  • Ion mobility spectrometry (IMS) offers rapid separation and structural characterization of ions.
  • However, limited sensitivity due to ion losses at the interface has restricted its widespread adoption in mass spectrometry (MS).

Purpose of the Study:

  • To develop a novel electrospray ionization-ion mobility spectrometry-quadrupole time-of-flight mass spectrometry (ESI-IMS-QTOF MS) instrument with enhanced sensitivity.
  • To overcome ion transmission losses at the ESI-IMS and IMS-QTOF interfaces.

Main Methods:

  • Incorporation of electrodynamic ion funnels, including a novel "hourglass" design, at the ESI-IMS and IMS-QTOF interfaces.
  • Utilizing radiofrequency (rf) ion focusing at the IMS terminus without compromising resolving power.

Related Experiment Videos

Main Results:

  • The new instrument achieves essentially lossless ion transmission across relevant ion masses, even with long drift tubes.
  • IMS resolving power exceeds 100 for singly charged ions, approaching theoretical limits.
  • The overall system sensitivity is comparable to commercial ESI-MS.

Conclusions:

  • The developed ESI-IMS-MS system significantly enhances sensitivity and ion transmission.
  • This advancement makes IMS-MS a viable technique for ultrahigh-sensitivity analysis of complex mixtures with high throughput.