Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microarray expression profiling identifies early signaling transcripts associated with 6-OHDA-induced dopaminergic

William A Holtz1, Jay M Turetzky, Karen L O'Malley

  • 1Washington University School of Medicine, Anatomy and Neurobiology Department, St. Louis, MO 63110, USA.

Antioxidants & Redox Signaling
|May 14, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activation of Endoplasmic Reticulum-Localized Metabotropic Glutamate Receptor 5 (mGlu<sub>5</sub>) Triggers Calcium Release Distinct from Cell Surface Counterparts in Striatal Neurons.

Biomolecules·2025
Same author

Location and Cell-Type-Specific Bias of Metabotropic Glutamate Receptor, mGlu<sub>5</sub>, Negative Allosteric Modulators.

ACS chemical neuroscience·2019
Same author

Intracellular GPCRs Play Key Roles in Synaptic Plasticity.

ACS chemical neuroscience·2018
Same author

GPCR signalling from within the cell.

British journal of pharmacology·2017
Same author

Sequences within the C Terminus of the Metabotropic Glutamate Receptor 5 (mGluR5) Are Responsible for Inner Nuclear Membrane Localization.

The Journal of biological chemistry·2017
Same author

Mechanisms Associated with Activation of Intracellular Metabotropic Glutamate Receptor, mGluR5.

Neurochemical research·2016

This study reveals how 6-hydroxydopamine (6-OHDA) triggers cellular stress and protein issues in Parkinson's disease models. It maps the molecular timeline of these neurotoxic events.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics

Background:

  • 6-hydroxydopamine (6-OHDA) is a parkinsonian mimetic.
  • 6-OHDA induces transcriptional changes linked to cellular stress and unfolded protein response.
  • Understanding toxin-mediated pathways is crucial for Parkinson's disease research.

Purpose of the Study:

  • To decipher toxin-mediated regulatory pathways using functional genomics and proteomics.
  • To identify distinct gene subgroups and their kinetic patterns following 6-OHDA treatment.
  • To provide a temporal understanding of molecular events in 6-OHDA neurotoxicity.

Main Methods:

  • Microarray analysis of RNA at multiple time points post-6-OHDA treatment.
  • Data mining and clustering techniques to identify gene subgroups.

Related Experiment Videos

  • Real-time PCR, 2D electrophoresis, and Western blotting for validation.

    • Main Results:

    • Robust upregulation of stress-induced transcription factors (ATF3, ATF4, CHOP, C/EBP beta) with unique kinetics.
    • Identification of distinct kinetic profiles for genes involved in protein synthesis, degradation, and oxidative stress.
    • Validation of microarray data through independent experimental methods.

    Conclusions:

    • Oxidative stress and protein dysfunction are implicated in Parkinson's disease pathogenesis.
    • The study provides a detailed time course of molecular events in 6-OHDA neurotoxicity.
    • Functional genomics and proteomics offer insights into neurodegenerative disease mechanisms.