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Quantitative and cytotoxic activity determinations on Galanthus nivalis subsp. cilicicus
1Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey. dumani@pharm.ege.edu.tr
Fitoterapia
|May 14, 2005
Summary
This study analyzed Galanthus nivalis subsp. cilicicus from Turkey, determining its chemical composition and alkaloid content. Findings provide data for potential monographs on this medicinal plant.
Area of Science:
- Pharmacognosy
- Ethnobotany
- Phytochemistry
Background:
- Galanthus nivalis subsp. cilicicus is a wild-growing species in Turkey.
- Traditional medicinal uses necessitate detailed chemical profiling.
Purpose of the Study:
- To establish quality control parameters for Galanthus nivalis subsp. cilicicus.
- To quantify key alkaloids: galanthamine and lycorine.
- To assess the bioactivity of extracts via brine shrimp lethality bioassay.
Main Methods:
- Macroscopic and microscopic analysis of Herba and Bulbus Galanthi.
- Determination of humidity, ash, and sulphated ash content (DAB 10).
- Quantitative analysis of galanthamine and lycorine using spectrophotometry and TLC.
- Brine shrimp lethality bioassay for ethanolic and alkaloidal extracts.
Main Results:
- Chemical composition and alkaloid profiles were determined for different vegetation periods.
- Galanthamine and lycorine content varied between flowering and fruiting seasons.
- LC50 values indicated differential toxicity of extracts.
Conclusions:
- The study provides essential data for the pharmacopoeial standardization of Galanthus nivalis subsp. cilicicus.
- Alkaloid content and bioactivity are influenced by the plant's phenological stage.
- Further research into the therapeutic potential of this species is warranted.