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Analysis of Xenopus melanotrope cell size and POMC-gene expression
G J H Corstens1, E W Roubos, B G Jenks
1Department of Cellular Animal Physiology, Radboud University Nijmegen, the Netherlands.
Abstract:
Flow cytometry was used to separate Xenopus melanotrope cells according to their size. The cells were then submitted to real-time RT-PCR to determine the level of POMC-gene expression. The results show a positive correlation between cell size and gene expression for cells from black-background (but not white-background) adapted animals.
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