Biomaterials functionalization using a novel peptide that selectively binds to a conducting polymer

Area of Science:

• Biomaterials Science
• Polymer Chemistry
• Molecular Biology

Background:

• Biomaterial surface modification aims to impart specific functions without changing bulk properties.
• Electrically conductive polymers, such as chlorine-doped polypyrrole (PPyCl), are promising for electronic and biomedical uses.
• Developing methods to selectively functionalize polymer surfaces is crucial for advanced applications.

Purpose of the Study:

• To develop a novel surface functionalization strategy for chlorine-doped polypyrrole (PPyCl) using phage display.
• To isolate and characterize peptides that specifically bind to PPyCl.
• To demonstrate the utility of the functionalized PPyCl for promoting cell attachment.

Main Methods:

• Phage display was employed to screen a library of 12-mer peptides against PPyCl.
• Binding phage (phiT59) and its peptide were isolated and characterized using fluorescence microscopy and titer count analysis.
• Atomic force microscopy and fluorescamine assays were used to study peptide binding affinity and mechanism.

Main Results:

• A specific PPyCl-binding phage (phiT59) and its corresponding 12-mer peptide were identified.
• The binding stability and specificity of the peptide to PPyCl were confirmed.
• The T59 peptide, when conjugated to a cell-adhesive sequence, successfully promoted cell attachment on PPyCl.

Conclusions:

• Phage display offers a versatile strategy for the specific surface functionalization of PPyCl.
• This approach allows for the development of bioactive materials by immobilizing various molecules onto PPyCl.
• The method is adaptable for other polymers, enabling the creation of tailored bioactive materials without compromising their intrinsic properties.