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Updated: Aug 18, 2026

Preparation of Poly(pentafluorophenyl acrylate) Functionalized SiO2 Beads for Protein Purification
Published on: November 19, 2018
Highly branched stimuli responsive poly[(N-isopropyl acrylamide)-co-(1,2-propandiol-3-methacrylate)]s with protein
Steven Carter1, Stephen Rimmer, Alice Sturdy
1The Polymer and Biomaterials Chemistry Laboratories, Department of Chemistry (Polymer Centre), Brook Hill, University of Sheffield, Sheffield, South Yorkshire, UK S3 7HF, United Kingdom.
Abstract:
Highly branched poly(NIPAM) have been prepared using the technique of reversible addition-fragmentation chain transfer (RAFT) polymerisation using a chain transfer agent that allows the incorporation of imidazole functionality in the polymer chain-ends. The lower critical solution temperature (LCST) of the polymers can be controlled by the amount of hydrophobe and GMA incorporated during copolymerisation procedures. These thermally responsive "smart" polymers were used to purify a His-tagged BRCA-1 protein fragment by affinity precipitation. [Diagram: see text]
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