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Published on: June 28, 2019
[A preliminary study on estimation of postmortem interval according to beta-actin mRNA stability in rat]
Jun-hui Xiao1, Yu-chuan Chen, Jiang-feng Wang
1Department of forensic pathology, preliminary medical college of Sun Yat-Sen University, Guangzhou 510080, China. xiaoxiaopost@163.com
Objective:
In order to find a new parameter to estimate the postmortem interval, beta-actin mRNA in lung and thoracic muscle of rats was detected at different time point postmortem.
Methods:
Rats were killed by neck dislocation and left in a temperature controlling system at 21 degrees C for 12 days postmortem. Total RNA in lung and thoracic muscle at different time point was extracted and beta-actin mRNA expression was examined by RT-PCR. Semi-quantification analysis of the image of electrophoresis was performed to confirm the changes of beta-actin mRNA expression.
Results:
beta-actin mRNA in lung of rats still could be detected at 12 days postmortem, but-disappeared in thoracic muscle at 8 days postmortem.
Conclusion:
The expression of beta-actin mRNA in lung and thoracic muscle could be a new parameter for estimation of PMI.
