Establishment of a novel human B-CLL-like xenograft model in nude mouse

We have developed a novel murine model for B-CLL by engrafting human prolymphocytic leukemia (PLL) or B-CLL cell line cells (JVM-3 and MEC-2 cell lines, respectively) into nude mice. Not only treatment of the mice was a prerequisite for the success of the graft, but also for the first time, females appeared to accept the cells more easily than males. Surprisingly, tumoral murine models for B-CLL could be established with PLL cells but not with B-CLL cells. JVM-3 cells were efficiently transplanted into nude mice through subcutaneous or intravenous routes. Irradiated female mice appeared to be the optimal recipients for tumor growth. Such murine models for human B-CLL may help the development of therapeutic agents.