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Published on: February 12, 2020

[Effect of nucleolin down-regulation on the proliferation and apoptosis in C2C12 cells]

Kang-kai Wang1, Lei Jiang, Shun-mei E

  • 1Department of Pathophysiology , Xiangya School of Medicine, Central South University, Changsha 410078, China.

Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences
|May 19, 2005
PubMed
Abstract

Insights

Down-regulating nucleolin inhibits C2C12 cell proliferation and induces apoptosis. This study clarifies nucleolin

Area of Science:

  • Molecular Biology
  • Cell Biology

Context:

  • Nucleolin is a protein involved in various cellular processes.
  • Understanding its role in muscle cell differentiation is crucial.

Purpose:

  • To investigate the effect of nucleolin on C2C12 cell proliferation and apoptosis.
  • To determine if inhibiting nucleolin impacts these cellular behaviors.

Summary:

  • Nucleolin expression was inhibited in C2C12 cells using antisense oligonucleotides.
  • Cellular proliferation was assessed using MTT assays.
  • Apoptosis was detected via flow cytometry and DNA ladder assays.
  • Significant inhibition of proliferation and increased apoptosis were observed upon nucleolin down-regulation.

Impact:

  • This research highlights nucleolin's role as a regulator of C2C12 cell proliferation and apoptosis.
  • Findings suggest nucleolin as a potential therapeutic target for conditions involving muscle cell growth or death.

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