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Related Experiment Videos

Monocyte viability on titanium and copper coated titanium.

Felicia Suska1, Christina Gretzer, Marco Esposito

  • 1Department of Biomaterials, Institute of Surgical Sciences, Sahlgrenska Academy at Göteborg University, Box 412, SE-405 30 Göteborg, Sweden. felicia.suska@biomaterials.gu.se

Biomaterials
|May 19, 2005
PubMed
Summary

Titanium (Ti) and copper (Cu) implant materials differentially affect human monocyte cell death. Ti promotes survival and cytokine release, while Cu induces rapid apoptosis and necrosis, impacting inflammatory responses.

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Area of Science:

  • Biomaterials Science
  • Immunology
  • Cell Biology

Background:

  • The role of apoptosis in the inflammatory response to implanted materials is not well understood.
  • Titanium (Ti) and copper (Cu) surfaces exhibit distinct cytotoxic potentials and in vivo outcomes.

Purpose of the Study:

  • To investigate the influence of Ti and Cu surfaces on human monocyte apoptosis and necrosis.
  • To analyze the production of cytokines (TNF-alpha and IL-10) and cell membrane injury markers in response to these materials.

Main Methods:

  • Human monocytes were incubated on Ti and Cu surfaces.
  • Annexin V/PI staining was used to discriminate apoptotic and necrotic cells.
  • Staurosporine was used as a positive control for apoptosis induction.
  • Lactate dehydrogenase (LDH) and cytokine levels were measured in culture medium.

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Main Results:

  • Ti surfaces enhanced monocyte survival and production of IL-10 and TNF-alpha.
  • Cu surfaces induced early signs of apoptosis in monocytes, progressing to apoptosis/necrosis within 48 hours.
  • Staurosporine treatment on Ti-adherent cells induced similar cell death patterns.
  • Low LDH and cytokine levels were observed around Cu surfaces, potentially due to ion interference.

Conclusions:

  • Material properties significantly and rapidly influence the onset and progression of human monocyte apoptosis and necrosis.
  • Early detection of apoptosis and cell death is crucial for understanding the biological response to implanted biomaterials.