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Volatile and polar compounds in Rosadamascena Mill 1803 cell suspension
Atanas Pavlov1, Simeon Popov, Elena Kovacheva
1Institute of Microbiology, Bulgarian Academy of Sciences, Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Maritza 26 Blvd., Plovdiv 4002, Bulgaria.
Abstract:
Studies were conducted on low molecular metabolites (volatiles and polar compounds) produced by Rosa damascena Mill 1803 cell suspension culture, cultivated under different regimes: as a free suspension (in flasks and in bioreactor) and in a two-phase system (in the presence of Amberlite XAD-4 as a second phase). It was established that the main groups of volatiles were hydrocarbons and free acids and their esters and only traces of terpenoids were found. The main components of polar fraction were free acids, especially amino acids and oxidized acids. Depending on the culture conditions, significant differences were established in the amounts of all compounds under study in biomasses, culture media and adsorbed on the second phase (Amberlite XAD-4).
