Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A stability indicating LC method for zolmitriptan.

B Mallikarjuna Rao1, M K Srinivasu, G Sridhar

  • 1Analytical Research, Custom Pharmaceutical Services, Dr. Reddy's Laboratories, Hyderabad 500049, India. bmrao@drreddys.com

Journal of Pharmaceutical and Biomedical Analysis
|May 19, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In Silico Guided Synthesis and Biological Evaluation of s-Triazine Derivatives as Potential Antibacterial Agents.

Recent advances in anti-infective drug discovery·2026
Same author

Nutritional and Performance Effects of Partial Replacement of Maize With Cassava Root (<i>Manihot esculenta</i> Crantz) Meal in Broiler Diets.

Food science & nutrition·2026
Same author

Comment on "Toward Timely Diagnosis of Pancreatic Cancer: Revolutionizing Early Detection Through Genomics, Artificial Intelligence, and Noninvasive Biomarkers".

Journal of gastroenterology and hepatology·2026
Same author

Comment on "Developing machine learning-enhanced WHODAS 2.0 short forms for persons with dementia".

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same author

Chemical and Mechanical Evolution of a Volumetric Additive Manufacturing (VAM) Resin Under High-Temperature Storage.

Polymers·2026
Same author

Effects of Visible and UV Illumination on Dimensional Accuracy and Surface Roughness in Dual-Color Volumetric Additive Manufacturing (VAM).

Materials (Basel, Switzerland)·2026

A new reversed-phase liquid chromatography (RP-LC) method quantifies zolmitriptan, an effective migraine treatment. This validated assay also determines related substances and impurities in bulk drugs, ensuring quality control.

Area of Science:

  • Analytical Chemistry
  • Pharmaceutical Analysis

Background:

  • Zolmitriptan is a crucial medication for severe migraine headaches.
  • Accurate quantification and impurity profiling are essential for pharmaceutical quality control.

Purpose of the Study:

  • To develop and validate a gradient, reversed-phase liquid chromatography (RP-LC) assay method for zolmitriptan.
  • To establish a method applicable for determining related substances and impurities in bulk zolmitriptan.

Main Methods:

  • Utilized a Waters X Terra RP18 column (250 mm x 4.6 mm, 5 µm).
  • Employed a gradient mobile phase consisting of phosphate buffer (pH 9.85), methanol, and acetonitrile.
  • Detected analytes at 225 nm with a flow rate of 1.0 mL/min.

Main Results:

Related Experiment Videos

  • Achieved resolution > 3 between zolmitriptan and its impurities (Imp-1, Imp-2, Imp-3).
  • Zolmitriptan showed significant degradation under alkaline and oxidative stress conditions.
  • Imp-3 was identified as the degradation product from alkaline hydrolysis.
  • Mass balance was consistently close to 99.5% across stress studies.

Conclusions:

  • The developed RP-LC method is suitable for the quantitative determination of zolmitriptan and its related substances.
  • The method demonstrates robustness and is validated for linearity, accuracy, and precision.
  • This assay is critical for ensuring the quality and safety of zolmitriptan in pharmaceutical formulations.