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Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination
Published on: August 24, 2015
[Bacterial contamination in blood and blood products]
Rüchan Yazan Sertöz1, Söhret Aydemir, Akgün Uysal
1Ege Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Izmir.
Abstract:
Skin disinfection during phlebotomy is a critical step for bacterial contamination of blood and blood products. The aim of this study was to investigate the bacterial contamination rates during phlebotomy and to detect the probable microorganisms present. Skin disinfections of 100 blood donors were performed by using povidone iodine solution with standard procedure. Fifteen mililiters of blood samples were drawn from the transfusion set and inoculated into culture flasks of automated Bact/Alert (BioMerieux) system. Blood cultures were monitorized for one week, and bacteria in positive cultures were identified by using classical microbiological methods in addition with API identification system (BioMerieux; ID32 Staph, 20 Strep). As a result, bacterial growth was detected in four (4%) of the blood samples, whereas 96% of the samples were found sterile. Staphylococcus epidermidis was the microorganism which had been grown in three of the samples, and Streptococcus mutans in one. The positivity rate detected in our study was considered high, since expected bacterial contamination rates in blood transfusions were between 0.2-0.5%. This data indicated that the procedures used in phlebotomy such as the choice of phlebotomy region, disinfectant use and disinfection time should be re-evaluated in our blood centre.
Insights
Bacterial contamination during blood collection occurred in 4% of donors, higher than expected. This suggests re-evaluating skin disinfection protocols in blood donation centers is crucial to ensure blood product safety.
Area of Science:
- Microbiology
- Transfusion Medicine
- Infectious Disease
Background:
- Skin disinfection during phlebotomy is essential to prevent bacterial contamination of blood and blood products.
- High contamination rates pose a significant risk to transfusion safety.
Purpose of the Study:
- To investigate bacterial contamination rates during phlebotomy.
- To identify microorganisms present in blood samples post-disinfection.
Main Methods:
- 100 blood donors underwent skin disinfection with povidone-iodine.
- Blood samples were collected and cultured using the automated Bact/Alert system.
- Positive cultures were analyzed using classical microbiology and API identification systems.
Main Results:
- Bacterial growth was detected in 4% of blood samples.
- Staphylococcus epidermidis (3 samples) and Streptococcus mutans (1 sample) were identified.
- The observed contamination rate (4%) significantly exceeded the expected rate (0.2-0.5%).
Conclusions:
- The current phlebotomy disinfection procedures may be inadequate.
- Re-evaluation of phlebotomy site selection, disinfectant efficacy, and disinfection time is recommended.
- Improving disinfection protocols is vital for enhancing blood product safety.
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