Related Experiment Video

Updated: Aug 18, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Cotranscriptional mRNP assembly: from the DNA to the nuclear pore

Andrés Aguilera1

  • 1Departamento de Genética, Facultad de Biología, Universidad de Sevilla, Avd. Reina Mercedes 6, 41012 Sevilla, Spain. aguilo@us.es

Current Opinion in Cell Biology
|May 20, 2005
PubMed

Insights

Messenger ribonucleoprotein (mRNP) particle assembly occurs during transcription, involving RNA-binding proteins that control mRNA fate. This genetically regulated process is crucial for gene expression and genome stability.

Area of Science:

  • Molecular Biology
  • Gene Expression Regulation
  • RNA Biology

Background:

  • Transcription is coupled with the assembly of RNA-binding proteins onto nascent mRNA.
  • This process forms stable, export-competent messenger ribonucleoprotein (mRNP) particles.
  • RNA-binding factors guide mRNA processing, localization, translation, and stability.

Purpose of the Study:

  • To elucidate the mechanisms and significance of mRNP particle assembly during transcription.
  • To highlight the role of RNA-binding proteins in controlling gene expression.
  • To explore the connection between mRNP assembly and genome integrity.

Main Methods:

  • Observational studies of transcription sites.
  • Analysis of RNA-binding protein recruitment dynamics.
  • Investigation of splicing-dependent complex formation (e.g., exon-junction complex).

Main Results:

  • mRNP assembly is initiated by cap-binding complexes and involves splicing-dependent recruitment of factors.
  • RNA-binding factors recruited during transcription dictate downstream mRNA processing and function.
  • Emerging evidence points to mRNP assembly as a genetically controlled pathway.

Conclusions:

  • mRNP assembly is a critical, coordinated process essential for producing functional mRNA.
  • This assembly pathway plays a significant role in regulating gene expression.
  • mRNP biogenesis is implicated in maintaining genome integrity, extending beyond mRNA metabolism.

More Related Videos

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis11:04

A Cell Free Assay to Study Chromatin Decondensation at the End of Mitosis

Published on: December 19, 2015

Related Concept Videos

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...