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Updated: Aug 18, 2026

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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
Published on: March 16, 2012
[Cloning and expression of a gene encoding shortened cecropin A-melittin hybrid in E.coli]
Jia-ming Zhu1, Fei-peng Liu, Yue-qin Li
1Department of Biotechnology, College of Life Scienceand Technology, Jinan University, Guangzhou 510630, China. tom@jnu.edu.cn
Yi Chuan = Hereditas
|May 20, 2005
Abstract:
According to the studies of David Andreu and other scientists, we designed and synthesized a gene encoding shortened cecropin A-melittin hybrid. This hybrid consists of CA(1-7) and M(5-12). The gene was inserted into pGEMEX-1 in the site of EcoRI and BamHI. A positive clone was obtained by hybridization and DNA sequencing and was expressed in JM109(DE3).

