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Morphologic characterization of specific granules in Greyhound eosinophils
1Veterinary Teaching Hospital and Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA. cline.180@osu.edu
Veterinary Clinical Pathology
|May 20, 2005
Summary
Vacuolated eosinophils in Greyhounds are likely due to differences in how specific granules stain with various hematologic stains. Ultrastructural and cytochemical analyses showed no significant differences between normal and vacuolated eosinophils in this breed.
Area of Science:
- Veterinary Hematology
- Canine Blood Cell Morphology
- Immunology
Background:
- Vacuolated eosinophils, characterized by nonstaining granules, are a known finding in Greyhounds.
- Previous studies have not thoroughly investigated the granule characteristics of these vacuolated eosinophils in this breed.
Purpose of the Study:
- To characterize the morphologic, ultrastructural, and cytochemical staining features of specific granules in both normal and vacuolated eosinophils in Greyhound blood.
- To compare these features between normal and vacuolated eosinophils within the Greyhound population.
Main Methods:
- Comparative analysis of peripheral blood smears from 49 Greyhounds and 200 non-Greyhound dogs using Wright's and Diff-Quik stains.
- Transmission electron microscopy (TEM) on blood samples from Greyhounds with both vacuolated and normal eosinophils.
- Cytochemical staining for alkaline phosphatase (AP), chloracetate esterase (CAE), and alpha naphthyl butyrate esterase (ANBE) on selected blood smears.
Main Results:
- Over half of Greyhounds (53%) exhibited vacuolated eosinophils with Wright's stain, compared to only 1% in other breeds.
- A significant proportion (85%) of Greyhounds with normal-appearing eosinophils on Wright's stain showed vacuolated eosinophils with Diff-Quik stain.
- TEM revealed no ultrastructural differences in granules between normal and vacuolated eosinophils.
- Both types of eosinophils in Greyhounds were positive for AP and negative for CAE and ANBE, consistent with normal canine eosinophils.
Conclusions:
- The presence of vacuolated eosinophils in Greyhounds is likely attributed to differential staining properties of their specific granules with common hematologic stains.
- Ultrastructural and cytochemical analyses indicate that the granules in vacuolated and normal eosinophils from Greyhounds are fundamentally similar.