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Updated: Feb 19, 2026

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
Published on: November 4, 2025
Quorum-sensing-based toolbox for regulatable transgene and siRNA expression in mammalian cells
Wilfried Weber1, Laetitia Malphettes, Matthias Rinderknecht
1Institute of Chemical- and Bio-Engineering, Swiss Federal Institute of Technology, ETH Hoenggerberg HCI F115, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
Abstract:
Technologies for regulated expression of multiple transgenes in mammalian cells have gathered momentum for bioengineering, gene therapy, drug discovery, and gene-function analyses. Capitalizing on recently developed mammalian transgene modalities (QuoRex) derived from Streptomyces coelicolor, we have designed a flexible and highly compatible expression vector set that enables desired transgene/siRNA control in response to the nontoxic butyrolactone SCB1. The construction-kit-like expression portfolio includes (i) multicistronic (pTRIDENT), (ii) autoregulated, (iii) bidirectional (pBiRex), (iv) oncoretro- and lentiviral transduction, and (v) RNA polymerase II-based siRNA transcription-fine-tuning vectors for straightforward implementation of QuoRex-controlled (trans)gene modulation in mammalian cells.
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