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Related Experiment Videos

PCI proteins eIF3e and eIF3m define distinct translation initiation factor 3 complexes.

Chunshui Zhou1, Fatih Arslan, Susan Wee

  • 1Department of Genetics and Complex Diseases, Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts 02115, USA. czhou@rics.bwh.harvard.edu

BMC Biology
|May 21, 2005
PubMed
Summary

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Fission yeast possesses two distinct eukaryotic translation initiation factor 3 (eIF3) complexes, eIF3e and eIF3m. These complexes, defined by PCI proteins, associate with different messenger RNA sets, impacting protein synthesis.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Protein Complexes

Background:

  • PCI/MPN domain protein complexes include the 19S proteasome lid, COP9 signalosome (CSN), and eukaryotic translation initiation factor 3 (eIF3).
  • eIF3 is believed to have essential core subunits for protein synthesis and non-essential subunits for mRNA specificity.
  • Previous studies noted interactions between eIF3 subunits and PCI proteins in the CSN.

Purpose of the Study:

  • To investigate the distinct roles of eIF3 complexes in fission yeast.
  • To characterize the novel eIF3m subunit and its relationship with eIF3e and the CSN.

Main Methods:

  • Comparative analysis of eIF3 complexes using genetic and molecular approaches in fission yeast.
  • Ribonomic analysis to identify associated mRNAs for each eIF3 complex.

Related Experiment Videos

  • RT-PCR validation of microarray findings for specific mRNAs.

    • Main Results:

    • Fission yeast exhibits two distinct eIF3 complexes, differentiated by PCI proteins eIF3e and a novel subunit, eIF3m.
    • eIF3m is essential for global protein synthesis and polysome formation, unlike eIF3e.
    • eIF3m associates with the majority of cellular mRNAs, while eIF3e binds a more restricted subset.

    Conclusions:

    • PCI proteins eIF3e and eIF3m define distinct eIF3 complexes in fission yeast.
    • These distinct complexes likely facilitate the translation of different mRNA populations.
    • This finding provides new insights into the regulation of protein synthesis by eIF3 complexes.