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Updated: Aug 17, 2026
![Mizoroki-Heck Cross-coupling Reactions Catalyzed by Dichloro{bis[1,1',1''-(phosphinetriyl)tripiperidine]}palladium Under Mild Reaction Conditions](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F51444.jpg&w=3840&q=50)
Mizoroki-Heck Cross-coupling Reactions Catalyzed by Dichloro{bis[1,1',1''-(phosphinetriyl)tripiperidine]}palladium Under Mild Reaction Conditions
Published on: March 20, 2014
Effect of Tris on catalytic activity of MP-11
Zhaopeng Qi1, Xi Li, Dongmei Sun
1Department of Chemistry, Nanjing Normal University, Nanjing 210097, People's Republic of China.
Abstract:
The effect of tris(hydroxymethyl)aminomethane (Tris) on the catalytic activity and microstructure of heme undecapeptide, microperoxidase-11 (MP-11) in the aqueous solution was investigated using cyclic voltammetry, circular dichroism (CD) spectroscopy, UV-vis absorption spectroscopy and X-ray photoelectron spectroscopy (XPS). It was found for the first time that Tris would inhibit the catalytic activity and electrochemical reaction of MP-11 at the glassy carbon (GC) electrode. This is mainly due to the fact that Tris would induce more alpha-helix and beta-turn conformations from the random coil conformation of MP-11, cause the asymmetric split-up in the Soret band region of MP-11, increase the non-planarity of the heme of MP-11, and change the electron densities of N, O and S atoms of MP-11. Meanwhile, It was found that the electrochemical reaction of MP-11 with Tris at GC electrode is diffusion-controlled, and the diffusion coefficient of MP-11 and the rate constant for the heterogeneous electron transfer of MP-11 in the presence of Tris are decreased by 19% and 16%, respectively. Further experiments showed that the electrocatalytic current of MP-11 on the reduction of H2O2 is decreased by about 25% after the addition of Tris to the MP-11 solution.
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