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Distinguishing different DNA heterozygotes by high-resolution melting

Robert Graham1, Michael Liew, Cindy Meadows

  • 1Department of Pathology, University of Utah School of Medicine, Salt Lake City 84132, USA.

Clinical Chemistry
|May 21, 2005
PubMed
Abstract

No abstract available in PubMed .

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

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