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Related Experiment Videos

Developmentally induced changes in transcriptional program alter spatial organization across chromosomes.

Jason M Casolari1, Christopher R Brown, David A Drubin

  • 1Department of Systems Biology, Harvard Medical School and The Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.

Genes & Development
|May 21, 2005
PubMed
Summary

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Yeast gene expression changes during mating involve relocating genes to the nuclear envelope. This nuclear organization shift is linked to mRNA export protein Mlp1 and depends on RNA and DNA elements.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Nuclear gene positioning influences transcriptional activity.
  • Dynamics of gene location during large-scale expression changes are poorly understood.
  • Yeast mating (Saccharomyces cerevisiae) triggers significant transcriptional and developmental shifts.

Purpose of the Study:

  • To investigate nuclear organization changes during yeast mating pheromone stimulus.
  • To identify factors and genomic elements involved in altered gene positioning.

Main Methods:

  • Stimulation of Saccharomyces cerevisiae with alpha factor (mating pheromone).
  • Analysis of gene association with nuclear envelope components.
  • Investigating the role of myosin-like protein Mlp1 and its RNA dependence.

Related Experiment Videos

  • High-resolution mapping of chromosome III association with Mlp1.

    • Main Results:

    • Most alpha-factor-induced genes associate with nuclear envelope components.
    • Mlp1's association with induced genes is RNA-dependent.
    • Identified alpha-factor-dependent determinants of nuclear pore association on chromosome III, including replication origins, intergenic regions, and 3' gene ends.

    Conclusions:

    • Reveals RNA- and DNA-dependent mechanisms governing nuclear organization.
    • Provides insights into large-scale chromosomal conformation changes in response to developmental cues.
    • Highlights the dynamic interplay between gene expression, nuclear architecture, and protein factors like Mlp1.