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Related Experiment Videos

Three developmental compartments involved in rib formation.

Hirohiko Aoyama1, Supyoko Mizutani-koseki, Haruhiko Koseki

  • 1Department of Anatomy and Developmental Biology, Graduate School of Medical Sciences, Hiroshima University, Minami-ku, Hiroshima, Japan. aoyamah@hiroshima-u.ac.jp.

The International Journal of Developmental Biology
|May 21, 2005
PubMed
Summary

Proximal rib development in chick embryos requires signals from the notochord and neural tube, potentially via Sonic hedgehog (Shh). Distal rib formation depends on the surface ectoderm, influencing Pax3 expression in the developing dermomyotome.

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Area of Science:

  • Developmental Biology
  • Embryology
  • Molecular Biology

Background:

  • Rib development is crucial for axial skeleton formation.
  • Somite derivatives are classified as primaxial and abaxial.
  • The precise signaling pathways governing rib compartmentalization remain incompletely understood.

Purpose of the Study:

  • To investigate the signaling requirements for proximal and distal rib development in chick embryos.
  • To elucidate the roles of axial tissues and surface ectoderm in rib formation.
  • To understand the contribution of Sonic hedgehog (Shh) signaling in rib development.

Main Methods:

  • Surgical manipulations in two-day chick embryos, including tissue separations using aluminum foil and polyethylene terephthalate film.
  • Co-transplantation experiments with notochord, ventral neural tube, and Shh-expressing cells.

Related Experiment Videos

  • Analysis of rib morphology and gene expression (Pax3) post-operation.

    • Main Results:

    • Separation of thoracic somitic mesoderm from the notochord/neural tube resulted in missing proximal rib parts.
    • Co-transplantation of notochord, ventral neural tube, or Shh-expressing cells rescued proximal rib development.
    • Separation from surface ectoderm led to missing distal rib parts and abnormal Pax3 expression, indicating dermomyotome defects.

    Conclusions:

    • Proximal rib development is dependent on the notochord and ventral neural tube, likely mediated by Shh signaling.
    • Distal rib development relies on signals from the surface ectoderm.
    • Ribs comprise three developmental compartments, aligning with primaxial and abaxial somite derivative classifications.