Bio/chemical analysis of dioxin-like compounds in sediment samples from Osaka Bay, Japan

The combinatorial bio/chemical investigation of sediments (six surface samples and one core sample) from Osaka Bay, Japan was conducted to clarify the horizontal and vertical distribution profiles of persistent organic pollutants in the sediments. Concentrations of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), polychlorinated biphenyls (PCBs), polybrominated dibenzo-p-dioxins and dibenzofurans (PBDD/Fs), and polybrominated diphenylethers (PBDEs) were determined by chemical analysis and compared to bioassay results using H4IIE-luc/Dioxin Responsive-Chemical Activated LUciferase eXpression (DR-CALUX). For surface sediments, World Health Organization-toxicity equivalent (WHO-TEQ) values ranged from 1.8 to 92 pg g(-1) dry weight and the bioassay-TEQ (CALUX-TEQ) values (3.7-140 pg g(-1) dry weight) yielded significant correlation with them (r2 = 0.96). On the other hand, correlation between both TEQs (for WHO-TEQ, 5.5-47 and for CALUX-TEQ, 27-76 pg g(-1) dry weight) for core samples was not so good (r2 = 0.46). Comparing the vertical profiles of CALUX-TEQ and WHO-TEQ, they were different in that WHO-TEQ reached the maximum in the 1957 core section, while CALUX-TEQ reached in the 1984 core section. CALUX-TEQ values were 1-5-fold more than WHO-TEQ values in all the surface and core samples. CALUX-TEQ values were calculated for PBDE and PBDD/F concentrations, employing their CALUX toxicity equivalent factors (CALUX-TEFs). The estimated CALUX-TEQ values obtained for the brominated compounds could explain for 11% on average (range 4.7-31%) of the experimentally obtained CALUX-TEQ values in the investigated surface sediments.