Related Experiment Video
Updated: Aug 17, 2026
Systematic Approach to Identify Novel Antimicrobial and Antibiofilm Molecules from Plants' Extracts and Fractions to Prevent Dental Caries
Published on: March 31, 2021
In vitro antibacterial activity of endodontic sealers
Giuseppe Pizzo1, Giovanni M Giammanco, Enzo Cumbo
1Department of Oral Sciences, University of Palermo, Via del Vespro 129, 90127 Palermo, Italy. giuseppepizzo@odonto.unipa.it
Objectives:
To evaluate the antibacterial activity of four endodontic sealers: one epoxy resin sealer (AH Plus), two zinc oxide eugenol (ZOE)-based sealers (Endomèthasone, Pulp Canal Sealer), and one sealer containing both ZOE and orthophenilphenol (Vcanalare).
Methods:
A direct contact test (DCT) was performed. A 10 microl suspension of Enterococcus faecalis was placed on the test material 20 min, 24 h and 7 days after mixing. Bacteria were allowed to directly contact the sealers for 1h at 37 degrees C. Bacterial growth was then spectrophotometrically measured every 30 min for 7 h, and again after 24 h as well.
Results:
All freshly mixed sealers showed complete inhibition of bacterial growth. Similar results were obtained with the 24-h-old samples, with the exception of AH Plus. Vcanalare was the only sealer still inhibiting bacterial growth 7 days after mixing.
Conclusions:
The antimicrobial activity of the tested sealers depends on the time interval between mixing and testing. All sealers exhibit bactericidal effect when freshly mixed, but only Vcanalare extended this effect until 7 days after setting.
