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Characterization of functional human erythrocyte-type glucose transporter (GLUT1) expressed in insect cells using a
C K Yi1, B M Charalambous, V C Emery
1Department of Biochemistry, Royal Free Hospital School of Medicine, University of London, U.K.
The Biochemical Journal
|May 1, 1992
Abstract:
The human erythrocyte-type glucose transporter (GLUT1) has been abundantly expressed in insect cells by using a recombinant baculovirus. At 4 days after infection with the virus, the insect cell-surface and intracellular membranes were found to contain greater than 200 pmol of D-glucose-sensitive binding sites for the transport inhibitor cytochalasin B per mg of protein. The characteristics of binding were identical with those of the erythrocyte transporter, although the two proteins differed substantially in apparent Mr, probably as a result of glycosylation differences.