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Published on: August 28, 2015
Analysis of diltiazem in Lipoderm transdermal gel using reversed-phase high-performance liquid chromatography applied
Jennifer L Buur1, Ronald E Baynes, James L Yeatts
1North Carolina State University, College of Veterinary Medicine, Center for Chemical Toxicology Research and Pharmacokinetics, 4700 Hillsborough St. Raleigh, NC 27606, USA. jib@cctrp.ncsu.edu
Abstract:
A simple and novel method for the extraction and quantification of diltiazem hydrochloride was developed and applied to homogenization and stability studies. The method used solid phase extraction coupled with reversed-phase high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. Validation showed inter-day recoveries ranging from 84.00 to 96.52% with relative standard deviations ranging from 12.01 to 15.94%. Intra-day recoveries ranged from 67.95 to 106.1% with relative standard deviations less than 5%. The method showed excellent linearity from 50 to 250 mg/ml in undiluted gel (R2 = 0.996). The homogenization study showed good homogenization using both 50 and 100 depression techniques. Diltiazem was stable at a concentration of 246 mg/ml for 30 days and at a concentration of 99.6 mg/ml for 60 days no matter the storage conditions explored in this study.
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