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alphaB-crystallin is phosphorylated during myocardial infarction: involvement of platelet-derived growth factor-BB

En Shu1, Hiroyuki Matsuno, Shigeru Akamastu

  • 1Department of Pharmacology, Gifu University Graduate School of Medicine, Japan.

Archives of Biochemistry and Biophysics
|May 24, 2005
PubMed

Insights

Platelet-derived growth factor (PDGF-BB) triggers alphaB-crystallin phosphorylation via p38 MAP kinase during heart attacks. This finding offers new insights into myocardial infarction treatment strategies.

Area of Science:

  • Cardiovascular Biology
  • Molecular Cardiology
  • Heat Shock Proteins

Background:

  • AlphaB-crystallin is a key heat shock protein in the heart, known for its cardioprotective effects during myocardial infarction.
  • Platelet-derived growth factor (PDGF) has shown promise in improving cardiac function post-myocardial infarction.

Purpose of the Study:

  • To investigate the phosphorylation of alphaB-crystallin during myocardial infarction.
  • To determine the role of Platelet-Derived Growth Factor-BB (PDGF-BB) in this process.

Main Methods:

  • Utilized a mouse model of myocardial infarction.
  • Measured alphaB-crystallin phosphorylation at Ser-59.
  • Assessed plasma PDGF-BB levels.
  • Employed SB203580, a p38 MAP kinase inhibitor, in cultured cardiac myocytes.

Main Results:

  • Phosphorylation of alphaB-crystallin at Ser-59 increased over time following myocardial infarction.
  • Plasma PDGF-BB levels were elevated concurrently with alphaB-crystallin phosphorylation.
  • PDGF-BB-induced phosphorylation was inhibited by SB203580, implicating p38 MAP kinase.

Conclusions:

  • PDGF-BB signaling pathway, mediated by p38 MAP kinase, is involved in alphaB-crystallin phosphorylation during myocardial infarction.
  • This mechanism may contribute to the cardioprotective roles of both alphaB-crystallin and PDGF-BB.

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