Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Leptin rapidly activates PPARs in C2C12 muscle cells.

Paola Bendinelli1, Roberta Piccoletti, Paola Maroni

  • 1Istituto di Patologia Generale, Università degli Studi di Milano, Italy.

Biochemical and Biophysical Research Communications
|May 24, 2005
PubMed
Summary

Leptin enhances gene expression in skeletal muscle by activating peroxisome proliferator-activated receptors (PPARs) via the ERK-cPLA2 pathway. This study reveals a direct hormonal effect on muscle transcription.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "miRNA-206 in muscle and central nervous system crosstalk during exercise: A double-edged sword with therapeutic potential" [Neurosci. Biobehav. Rev. 183 (2026) 106569].

Neuroscience and biobehavioral reviews·2026
Same author

Breast cancer bone metastasis and bone metastatic cells retain NKG2DLs intracellularly: could this be a strategy to evade immune recognition?

Frontiers in cell and developmental biology·2026
Same author

miRNA-206 in muscle and central nervous system crosstalk during exercise: A double-edged sword with therapeutic potential.

Neuroscience and biobehavioral reviews·2026
Same author

Caco2/HT-29 In Vitro Cell Co-Culture: Barrier Integrity, Permeability, and Tight Junctions' Composition During Progressive Passages of Parental Cells.

Biology·2025
Same author

Ex Vivo, In Vitro and In Vivo Bone Health Properties of Grana Padano Cheese.

Foods (Basel, Switzerland)·2025
Same author

Aberrant promoter methylation, expression and function of RASSF1A gene in a series of Italian parathyroid tumors.

Endocrine·2024

Area of Science:

  • Molecular Biology
  • Cellular Physiology
  • Endocrinology

Background:

  • Leptin is a hormone known to regulate energy balance and metabolism.
  • Evidence suggests leptin influences gene expression in various tissues, including skeletal muscle.
  • Peroxisome proliferator-activated receptors (PPARs) are key regulators of gene expression involved in metabolism.

Purpose of the Study:

  • To investigate the molecular mechanisms by which leptin modulates gene expression in skeletal muscle cells.
  • To determine if leptin's effect on skeletal muscle involves PPARs.
  • To elucidate the signaling pathway mediating leptin's action on PPARs.

Main Methods:

  • Electrophoretic mobility shift assays (EMSA) to assess DNA-binding activity of PPARs.
  • Treatment of C2C12 myoblasts with leptin and specific inhibitors (AACOCF3, PD-98059).

Related Experiment Videos

  • Measurement of cytosolic phospholipase A2 (cPLA2) activity and phosphorylation.
  • Evaluation of ERK pathway activation.

    • Main Results:

    • Leptin treatment increased the binding of C2C12 nuclear extracts to PPAR response elements.
    • All three PPAR isoforms were found to participate in leptin-induced DNA-binding complexes.
    • Inhibition of cPLA2 (cytosolic phospholipase A2) abrogated leptin-induced PPAR DNA-binding activity.
    • Leptin significantly increased cPLA2 activity and phosphorylation, mediated through ERK induction.

    Conclusions:

    • Leptin directly affects skeletal muscle cells.
    • Leptin modulates muscle transcription through the early activation of PPARs.
    • The ERK-cPLA2 signaling pathway is crucial for leptin-induced PPAR activation in skeletal muscle.