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Updated: Aug 17, 2026

Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
Published on: October 1, 2016
New principle of electromagnetophoretic adsorption-desorption microchromatography
Yoshinori Iiguni1, Hitoshi Watarai
1Department of Chemistry, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
Abstract:
A new principle for the chromatographic micro-separation of micrometer-sized particles in liquid has been invented by switching the electromagnetophoretic force in a capillary flow system. The principle is the combination of the Stokes force by the bulk flow and the adsorption-desorption force on a capillary inner surface controlled by an electromagnetophoretic buoyancy generated by an alternative current and a homogenous magnetic field. The observed retention profiles of test micro-particles was explained by the "zigzag" migration model mainly depended on particle size and their adsorption force to the capillary wall. By this method, we could succeed to separate polystyrene particles of 10 microm and 20 microm in diameter dispersed in 1 M KCl solution containing 0.01% Triton X-100 using only 1 mm long fused-silica capillary under 10T.
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