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Updated: Aug 17, 2026
Using Caco-2 Cells to Study Lipid Transport by the Intestine
Published on: August 20, 2015
Application of HPLC for determination of phytic acid in the colonic epithelial Caco-2 cells
Ludmiła Weglarz1, Beata Parfiniewicz, Beata Bat
1Department of Biochemistry, Medical University of Silesia 1 Narcyzów Str., 41-200 Sosnowiec, Poland.
Abstract:
Phytic acid (myo-inositol hexaphosphate, IP6) is currently receiving a considerable interest because of its anticancer (preventive and therapeutic) potential against colon tumors and the need for methods of its determination. The aim of this study was to analyze the uptake of IP6 by human colon adenocarcinoma cells (Caco-2 cell line) and to evaluate the method of its intracellular quantification with the use of high performance liquid chromatography (HPLC) technique. Chromatographic analysis revealed a rapid uptake of IP6 by cells. The intracellular accumulation of IP6 was saturable at 0.5 h and it did not change with the prolongation of incubation up to 72 h.
