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Related Experiment Videos

Clonality among Enterococcus faecium clinical isolates.

L Baldassarri1, L Bertuccini, R Creti

  • 1Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Università Campus Biomedico, Rome, Italy. baldassa@iss.it

Microbial Drug Resistance (Larchmont, N.Y.)
|May 25, 2005
PubMed
Summary

A specific clone of glycopeptide-resistant Enterococcus faecium emerged in a Rome hospital, showing increased resistance and adaptation. This finding highlights the rapid spread of resistant strains in clinical settings.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Epidemiology

Background:

  • Enterococcal infections pose a significant threat in healthcare settings.
  • The emergence of glycopeptide resistance in Enterococcus faecium is a growing concern.
  • Understanding the epidemiology and characteristics of resistant strains is crucial for infection control.

Purpose of the Study:

  • To investigate a sudden increase in glycopeptide-resistant Enterococcus faecium (GRE) at a university hospital in Rome.
  • To characterize the clonal relatedness, resistance genes, and virulence factors of isolated GRE strains.
  • To identify the factors contributing to the persistence and spread of GRE in a clinical environment.

Main Methods:

  • Epidemiological survey of enterococcal infections over one year.

Related Experiment Videos

  • Pulsed-field gel electrophoresis (PFGE) for assessing clonal relatedness.
  • Detection of vancomycin-resistance genes and virulence factors (e.g., esp gene).
  • Assessment of aminoglycoside resistance and biofilm formation.

    • Main Results:

    • A single pulsed-field type dominated the glycopeptide-resistant Enterococcus faecium isolates, indicating clonal spread.
    • Most resistant isolates exhibited high-level aminoglycoside resistance and were non-biofilm forming.
    • Two esp gene-carrying strains were found, but belonged to different PFGE types.
    • A specific clone acquired glycopeptide resistance in the clinical setting, enhancing its adaptability.

    Conclusions:

    • A specific clone of glycopeptide-resistant Enterococcus faecium has successfully adapted and spread within the hospital.
    • The acquisition of glycopeptide resistance by this clone facilitated its environmental persistence.
    • This study underscores the importance of molecular surveillance for tracking and controlling the spread of resistant pathogens in healthcare facilities.