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Growth at high pH increases Enterococcus faecalis adhesion to collagen.

G Kayaoglu1, H Erten, D Ørstavik

  • 1Department of Endodontics and Conservative Treatment, Faculty of Dentistry, Gazi University, Ankara, Turkey. guvenk@gazi.edu.tr

International Endodontic Journal
|May 25, 2005
PubMed
Summary

Alkaline conditions increase Enterococcus faecalis adherence to collagen, potentially explaining its persistence in root canals. Growth at higher pH enhances bacterial binding to collagen type I, but reduces binding to bovine serum albumin (BSA).

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Area of Science:

  • Microbiology
  • Biochemistry
  • Endodontics

Background:

  • Enterococcus faecalis is a common pathogen in persistent endodontic infections.
  • The role of environmental pH in E. faecalis virulence factors, such as adherence, is not fully understood.

Purpose of the Study:

  • To investigate the impact of varying pH levels (7.1-9.5) on the in vitro adherence of Enterococcus faecalis to bovine serum albumin (BSA) and collagen type I.

Main Methods:

  • Enterococcus faecalis strain A197A was cultured in media adjusted to pH levels between 7.1 and 9.5.
  • Bacterial adherence to surfaces coated with BSA or collagen type I was quantified using crystal violet staining and spectrophotometry.
  • Statistical analysis included the Kolmogorov-Smirnov test, Levene's test, and Student's t-test (alpha = 0.05).

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Main Results:

  • E. faecalis adherence to BSA decreased as the growth medium's alkalinity increased; bacteria grown at pH 7.1 showed significantly higher BSA binding.
  • Conversely, bacterial adherence to collagen type I significantly increased for bacteria grown at pH 8.0 and 8.5 compared to pH 7.1.

Conclusions:

  • A moderate increase in pH (up to 8.5) enhances the collagen-binding ability of E. faecalis.
  • This increased collagen adherence may be a key factor in E. faecalis dominance in persistent endodontic infections, possibly linked to alkaline medicament use.