Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracellular trypsin increases ASIC1a selectivity for monovalent versus divalent cations.

Emil Neaga1, Bogdan Amuzescu, Cristina Dinu

  • 1Department of Biophysics and Physiology, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, Bucharest 76201, Romania.

Journal of Neuroscience Methods
|May 25, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New Discoveries in the Field of Neuropharmacology.

Biomolecules·2026
Same author

3D Bioprinting of Blood Vessel Model for Improving Wound Healing.

International journal of molecular sciences·2026
Same author

Expression of serotonin transporter (SERT) and receptors 5-HTR1A and 5-HTR2A in an animal model of hypothermia.

Neuroscience letters·2025
Same author

Novel Micro-LC-MS/MS Method for the Quantification of Tenofovir and Its Active Metabolite Tenofovir-Diphosphate in Biological Matrices for Therapeutic Drug Monitoring.

Pharmaceuticals (Basel, Switzerland)·2025
Same author

Pharmaceutical Contaminants Occurrence and Ecological Risk Assessment Along the Romanian Black Sea Coast.

Toxics·2025
Same author

Fiber-optic-guided near-infrared laser exposure induces depolarization of cultured primary sensory neurons and modifies biophysical properties of human Nav1.5 channels.

Journal of photochemistry and photobiology. B, Biology·2025

Extracellular trypsin or high calcium exposure can improve acid-sensing ion channel (ASIC) current reproducibility. These treatments reduce persistent current components, aiding pharmacological studies of ASIC channels.

Area of Science:

  • Neuroscience
  • Ion Channel Physiology

Background:

  • Sustained proton activation of acid-sensing ion channels (ASICs) in neurons and HEK293 cells causes current rundown and a persistent component.
  • This rundown hinders accurate titration experiments in pharmacological studies of ASICs.

Purpose of the Study:

  • To identify methods to alleviate the persistent component of ASIC currents.
  • To improve the reproducibility of acid-elicited ASIC transients for better pharmacological characterization.

Main Methods:

  • Treatment of cells with extracellular trypsin or high extracellular calcium.
  • Measurement of transient inward currents using patch-clamp electrophysiology.
  • Selectivity measurements in bi-ionic conditions to assess ion permeability changes.

Related Experiment Videos

Main Results:

  • Extracellular trypsin (10-45 microg/ml for 5 min) and high Ca2+ (75 mM for <1 min) reduced the persistent current component.
  • These treatments significantly altered hASIC1a selectivity, decreasing divalent cation permeability relative to monovalent cations (P(Na)/P(Ca) increased).
  • Trypsin treatment lowered the slope conductance of the unit inward Ca2+ transient.

Conclusions:

  • Trypsin and high calcium exposure are effective in mitigating ASIC current rundown.
  • These treatments enhance the reliability of acid-elicited ASIC currents, facilitating pharmacological investigations.
  • The observed changes in ion selectivity suggest a mechanism for improving ASIC channel experimental reproducibility.