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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Published on: September 11, 2022
A new reversion assay for measuring all possible base pair substitutions in Saccharomyces cerevisiae
Teresa-Marie Williams1, Rebecca M Fabbri, Jason W Reeves
1Department of Biology, Emory University, Atlanta, Georgia 30322, USA.
Genetics
|May 25, 2005
Abstract:
A TRP5-based reversion system that allows the rates of all possible base pair substitutions to be measured when the TRP5 locus is in both orientations relative to a defined origin of replication has been developed. This system should be useful for a wide variety of mutation and repair studies in yeast.
