Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro micronucleus technique to predict chemosensitivity.

Michael Fenech1

  • 1CSIRO Health Science and Nutrition, South Australia, Australia.

Methods in Molecular Medicine
|May 25, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using effect biomarker thresholds in regulatory risk assessment: an OECD-based framework for assessing cumulative exposures to chemical mixtures.

Environment international·2026
Same author

Ageing phenotypes and behavioural determinants of health on age acceleration: the Cancer Survivors' Trajectories of Ageing Research (C*STAR) study protocol.

BMJ open·2026
Same author

The Buccal Micronucleus (BMN) Cytome assay: enhancing its applications by addressing challenges and exploring new opportunities.

Mutagenesis·2026
Same author

High-dose vitamin D supplementation and prostate cancer progression: a phase II randomised trial in localised prostate cancer cases with intermediate risk of progression (ProsD).

British journal of cancer·2025
Same author

Comparative Analysis of Aging-related Metabolic and Biological Markers between Cancer Survivors and Healthy Populations: A Systematic Review.

Journal of cancer prevention·2025
Same author

Innovating the buccal micronucleus cytome assay to improve its utility as a biomarker of in vivo genotoxicity.

Mutagenesis·2025

The cytokinesis-block micronucleus (CBMN) assay reliably measures chromosome damage, including breakage and loss, in genetic toxicology studies. This method enhances precision by analyzing binucleate cells, aiding in genotoxicity screening and radiosensitivity prediction.

Area of Science:

  • Genetic Toxicology
  • Cell Biology
  • Molecular Biology

Background:

  • Chromosomal mutations are critical in carcinogenesis.
  • Micronucleus assays are preferred for assessing chromosome damage, measuring loss and breakage.
  • The cytokinesis-block micronucleus (CBMN) assay improves precision by analyzing binucleate cells, avoiding confounding factors from cytotoxicity or suboptimal culture conditions.

Purpose of the Study:

  • To describe the principles and methods of the CBMN assay for assessing genotoxicity and cytotoxicity.
  • To highlight the CBMN assay's application in population monitoring, chemical screening, and radiosensitivity prediction.
  • To identify areas for future development of the CBMN assay.

Main Methods:

  • Utilizing cytochalasin-B to block cytokinesis, resulting in binucleate cells.

Related Experiment Videos

  • Analyzing binucleate cells for micronuclei formation to assess chromosome breakage and loss.
  • Employing modifications like cytosine-arabinoside treatment and molecular probes for detailed analysis of DNA damage and repair.

    • Main Results:

    • The CBMN assay provides multiple measures of genotoxicity and cytotoxicity, including chromosome breakage, loss, rearrangement, cell division inhibition, necrosis, and apoptosis.
    • The assay allows for the measurement of excision-repairable lesions with cytosine-arabinoside modification.
    • Molecular probes enable differentiation between chromosome loss and breakage, and measurement of nondisjunction.

    Conclusions:

    • The in vitro CBMN assay offers a versatile and relatively simple system for multiple genotoxicity and cytotoxicity assessments.
    • The CBMN assay is a valuable tool for various applications, from chemical screening to clinical predictions.
    • Further development of the CBMN assay holds promise for advancing our understanding of genetic damage and its consequences.