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A test for ectopic exchange catalyzed by Cre recombinase in maize
Thomas S Ream1, Jonathan Strobel, Brandon Roller
1Division of Biological Sciences, University of Missouri-Columbia, Columbia, 65211, USA.
Summary
Cre recombinase activity in maize was investigated for potential genomic rearrangements. Studies found no evidence of ectopic recombination or chromosomal aberrations in maize expressing Cre recombinase.
Area of Science:
- Genetics
- Molecular Biology
- Plant Science
Background:
- Cre recombinase is a tool for genetic engineering.
- Understanding its activity in maize is crucial for its application.
Purpose of the Study:
- To assess the potential for ectopic recombination in maize induced by Cre recombinase.
- To evaluate the safety and efficacy of Cre recombinase for genetic manipulation in maize.
Main Methods:
- Assaying maize lines expressing Cre recombinase and control lines.
- Performing pollen examination and seed set tests.
- Conducting root-tip chromosome karyotype analysis.
Main Results:
- No evidence of ectopic recombination was found in maize expressing Cre recombinase.
- Pollen viability and seed set were not adversely affected by Cre expression.
- Chromosome analysis revealed no significant chromosomal aberrations above background levels.
Conclusions:
- Cre recombinase does not induce ectopic recombination or chromosomal aberrations in maize.
- The use of Cre recombinase in maize is considered safe for genetic engineering applications.