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Updated: Aug 17, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
Analytical assessment of MALDI-TOF Imaging Mass Spectrometry on thin histological samples. An insight in proteome
Gianluca Maddalo1, Francesca Petrucci, Manuela Iezzi
1Centro Studi sull'Invecchiamento (Ce.S.I.), Fondazione Università "G.D'Annunzio", Chieti, Italy.
Background:
The development of MALDI Imaging Mass Spectrometry is a promising technique in the investigation of biological molecular repertoire. We have pursued an analytical assessment of this technique in its application to proteome analysis.
Methods:
A specific statistical method of analysis has been developed to enable data processing in the absence of internal standards, by defining similarity scores.
Results:
The investigated linear mode MALDI-TOF set-up allows to obtain data variations comprised within the 30% of variation when assaying tissues samples from the same animal, while the 60% of variation was highlighted in the inter-mice series assaying syngenic animals.
Conclusions:
This analytical assessment represents the first step of a process that should validate the utilisation of this technique in the clinical practice.
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