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Cholesteryl ester transfer protein in human brain.

J J Albers1, J H Tollefson, G Wolfbauer

  • 1Department of Medicine, University of Washington, Seattle.

International Journal of Clinical & Laboratory Research
|January 11, 1992
PubMed
Summary
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The brain synthesizes cholesteryl ester transfer protein (CETP) in the cerebrospinal fluid, indicating an active lipid transport system within the central nervous system. This protein is crucial for lipid redistribution in the brain.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Lipid Metabolism

Background:

  • Apolipoproteins and low-density lipoprotein receptors in the brain suggest an active lipid transport system.
  • Cholesteryl ester transfer protein (CETP) in plasma mediates lipid exchange among lipoproteins.

Purpose of the Study:

  • To investigate the presence and origin of cholesteryl ester transfer activity in human cerebrospinal fluid (CSF).
  • To determine if CETP is synthesized within the brain.

Main Methods:

  • Measurement of cholesteryl ester transfer activity in CSF and plasma from neurologically normal subjects.
  • Inhibition of CSF activity using a specific antibody against plasma CETP.
  • Analysis of CETP activity in conditioned media from brain cells and choroid plexus.

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Main Results:

  • Cholesteryl ester transfer activity was detected in CSF and was abolished by a specific CETP antibody.
  • CSF cholesteryl ester transfer activity was approximately 12% of plasma activity, while albumin was only 0.6%, suggesting local synthesis.
  • CETP activity was found in conditioned media from human neuroblastoma, neuroglioma cells, and sheep choroid plexus.

Conclusions:

  • Cholesteryl ester transfer protein is synthesized and secreted within the brain.
  • CETP likely plays a significant role in lipid transport and redistribution in the central nervous system.